| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This human FLOT2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
FLOT2 |
| Gene ID: |
2319 |
| Uniprot ID: |
FLOT2_HUMAN |
| Specificity: |
This kit recognizes Human FLOT2 in samples. No significant cross-reactivity or interference between Human FLOT2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes. |
| Post Translational Modifications | ZDHHC5-catalyzed palmitoylation predominantly occurs at Cys-4. ZDHHC5-catalyzed palmitoylation may be required for the formation of higher-order complexes and for neurite outgrowth in cultured neural stem cells. |
| Function | May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function. |
| Protein Name | Flotillin-2Epidermal Surface AntigenEsaMembrane Component Chromosome 17 Surface Marker 1 |
| Database Links | Reactome: R-HSA-5213460Reactome: R-HSA-5675482Reactome: R-HSA-8849932Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106Reactome: R-HSA-9696264Reactome: R-HSA-9696273 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinMembraneCaveolaEndosomeLipid-AnchorMembrane-Associated Protein Of Caveolae |
| Alternative CLIA Names | Flotillin-2 CLIA kitEpidermal Surface Antigen CLIA kitEsa CLIA kitMembrane Component Chromosome 17 Surface Marker 1 CLIA kitFLOT2 CLIA kitESA1 CLIA kitM17S1 CLIA kit |
| Specificity | This kit recognizes Human FLOT2 in samples. No significant cross-reactivity or interference between Human FLOT2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 97-112 | 103 |
| EDTA plasma(n=8) | 96-110 | 104 |
| Cell culture media(n=8) | 100-115 | 106 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.49 | 0.87 | 4.26 | 0.45 | 0.84 | 3.95 |
| Standard deviation | 0.04 | 0.10 | 0.39 | 0.04 | 0.09 | 0.38 |
| CV (%) | 8.16 | 11.49 | 9.15 | 8.89 | 10.71 | 9.62 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
