| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human FLNB kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
FLNB |
| Gene ID: |
2317 |
| Uniprot ID: |
FLNB_HUMAN |
| Specificity: |
This kit recognizes Human FLN Beta in samples. No significant cross-reactivity or interference between Human FLN Beta and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Ubiquitous. Isoform 1 and isoform 2 are expressed in placenta, bone marrow, brain, umbilical vein endothelial cells (HUVEC), retina and skeletal muscle. Isoform 1 is predominantly expressed in prostate, uterus, liver, thyroid, stomach, lymph node, small intestine, spleen, skeletal muscle, kidney, placenta, pancreas, heart, lung, platelets, endothelial cells, megakaryocytic and erythroleukemic cell lines. Isoform 2 is predominantly expressed in spinal cord, platelet and Daudi cells. Also expressed in thyroid adenoma, neurofibrillary tangles (NFT), senile plaques in the hippocampus and cerebral cortex in Alzheimer disease (AD). Isoform 3 and isoform 6 are expressed predominantly in lung, heart, skeletal muscle, testis, spleen, thymus and leukocytes. Isoform 4 and isoform 5 are expressed in heart. |
| Post Translational Modifications | ISGylation prevents ability to interact with the upstream activators of the JNK cascade and inhibits IFNA-induced JNK signaling. Ubiquitination by a SCF-like complex containing ASB2 isoform 1 leads to proteasomal degradation which promotes muscle differentiation. |
| Function | Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro. |
| Protein Name | Filamin-BFln-BAbp-278Abp-280 HomologActin-Binding-Like ProteinBeta-FilaminFilamin Homolog 1Fh1Filamin-3Thyroid AutoantigenTruncated Actin-Binding ProteinTruncated Abp |
| Database Links | Reactome: R-HSA-1169408 |
| Cellular Localisation | Isoform 1: CytoplasmCell CortexCytoplasmCytoskeletonStress FiberMyofibrilSarcomereZ LineIn Differentiating MyotubesIsoform 1Isoform 2 And Isoform 3 Are Localized Diffusely Throughout The Cytoplasm With Regions Of Enrichment At The Longitudinal Actin Stress FiberIn Differentiated TubesIsoform 1 Is Also Detected Within The Z-LinesIsoform 2: CytoplasmIsoform 3: CytoplasmIsoform 6: CytoplasmPolarized At The Periphery Of Myotubes |
| Alternative CLIA Names | Filamin-B CLIA kitFln-B CLIA kitAbp-278 CLIA kitAbp-280 Homolog CLIA kitActin-Binding-Like Protein CLIA kitBeta-Filamin CLIA kitFilamin Homolog 1 CLIA kitFh1 CLIA kitFilamin-3 CLIA kitThyroid Autoantigen CLIA kitTruncated Actin-Binding Protein CLIA kitTruncated Abp CLIA kitFLNB CLIA kitFLN1L CLIA kitFLN3 CLIA kitTABP CLIA kitTAP CLIA kit |
| Specificity | This kit recognizes Human FLNβ in samples. No significant cross-reactivity or interference between Human FLNβ and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-104 | 99 |
| EDTA plasma(n=8) | 86-100 | 92 |
| Cell culture media(n=8) | 99-113 | 106 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 219.46 | 549.44 | 1535.63 | 221.24 | 523.93 | 1556.80 |
| Standard deviation | 18.17 | 60.49 | 175.06 | 21.55 | 39.40 | 153.97 |
| CV (%) | 8.28 | 11.01 | 11.40 | 9.74 | 7.52 | 9.89 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
