• Human CTSB (Cathepsin B) CLIA Kit (STJC0000018)

Human CTSB (Cathepsin B) CLIA Kit (STJC0000018)

SKU:
STJC0000018

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human CTSB kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CTSB
Gene ID: 1508
Uniprot ID: CATB_HUMAN
Specificity: This kit recognizes Human CTSB in samples. No significant cross-reactivity or interference between Human CTSB and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum.
Function Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen. Has also been implicated in tumor invasion and metastasis.
Protein Name Cathepsin B
App Secretase
Apps
Cathepsin B1 Cleaved Into - Cathepsin B Light Chain - Cathepsin B Heavy Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1679131
Reactome: R-HSA-2022090
Reactome: R-HSA-2132295
Reactome: R-HSA-6798695
Cellular Localisation Lysosome
Melanosome
Secreted
Extracellular Space
Apical Cell Membrane
Peripheral Membrane Protein
Extracellular Side
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Localizes To The Lumen Of Thyroid Follicles And To The Apical Membrane Of Thyroid Epithelial Cells
Alternative CLIA Names Cathepsin B CLIA kit
App Secretase CLIA kit
Apps CLIA kit
Cathepsin B1 Cleaved Into - Cathepsin B Light Chain - Cathepsin B Heavy Chain CLIA kit
CTSB CLIA kit
CPSB CLIA kit
Specificity This kit recognizes Human CTSB in samples. No significant cross-reactivity or interference between Human CTSB and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 98-111 104
EDTA plasma(n=8) 101-116 108
Cell culture media(n=8) 100-114 106

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 100.33 277.70 727.18 92.77 304.35 793.10
Standard deviation 8.14 28.52 62.46 11.96 22.92 89.54
CV (%) 8.11 10.27 8.59 12.89 7.53 11.29
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance