| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human FCN1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
FCN1 |
| Gene ID: |
2219 |
| Uniprot ID: |
FCN1_HUMAN |
| Specificity: |
This kit recognizes Human FCN1 in samples. No significant cross-reactivity or interference between Human FCN1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Peripheral blood leukocytes, monocytes and granulocytes. Also detected in spleen, lung, and thymus, may be due to the presence of tissue macrophages or trapped blood in these tissues. Not detected on lymphocytes. |
| Function | Extracellular lectin functioning as a pattern-recognition receptor in innate immunity. Binds the sugar moieties of pathogen-associated molecular patterns (PAMPs) displayed on microbes and activates the lectin pathway of the complement system. May also activate monocytes through a G protein-coupled receptor, FFAR2, inducing the secretion of interleukin-8/IL-8. Binds preferentially to 9-O-acetylated 2-6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage. |
| Protein Name | Ficolin-1Collagen/Fibrinogen Domain-Containing Protein 1Ficolin-AFicolin-AlphaM-Ficolin |
| Database Links | Reactome: R-HSA-166662Reactome: R-HSA-166663Reactome: R-HSA-2855086Reactome: R-HSA-6798695 |
| Cellular Localisation | SecretedCell MembranePeripheral Membrane ProteinExtracellular SideFound On The Monocyte And Granulocyte Surface |
| Alternative CLIA Names | Ficolin-1 CLIA kitCollagen/Fibrinogen Domain-Containing Protein 1 CLIA kitFicolin-A CLIA kitFicolin-Alpha CLIA kitM-Ficolin CLIA kitFCN1 CLIA kitFCNM CLIA kit |
| Specificity | This kit recognizes Human FCN1 in samples. No significant cross-reactivity or interference between Human FCN1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-97 | 92 |
| EDTA plasma(n=8) | 98-111 | 104 |
| Cell culture media(n=8) | 85-99 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 94.89 | 248.25 | 947.31 | 94.87 | 250.57 | 976.47 |
| Standard deviation | 10.68 | 29.47 | 104.01 | 8.07 | 27.79 | 104.29 |
| CV (%) | 11.26 | 11.87 | 10.98 | 8.51 | 11.09 | 10.68 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
