• Human FCN1 (Ficolin-1) CLIA Kit (STJC0000226)

Human FCN1 (Ficolin-1) CLIA Kit (STJC0000226)

SKU:
STJC0000226

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human FCN1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: FCN1
Gene ID: 2219
Uniprot ID: FCN1_HUMAN
Specificity: This kit recognizes Human FCN1 in samples. No significant cross-reactivity or interference between Human FCN1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Peripheral blood leukocytes, monocytes and granulocytes. Also detected in spleen, lung, and thymus, may be due to the presence of tissue macrophages or trapped blood in these tissues. Not detected on lymphocytes.
Function Extracellular lectin functioning as a pattern-recognition receptor in innate immunity. Binds the sugar moieties of pathogen-associated molecular patterns (PAMPs) displayed on microbes and activates the lectin pathway of the complement system. May also activate monocytes through a G protein-coupled receptor, FFAR2, inducing the secretion of interleukin-8/IL-8. Binds preferentially to 9-O-acetylated 2-6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage.
Protein Name Ficolin-1
Collagen/Fibrinogen Domain-Containing Protein 1
Ficolin-A
Ficolin-Alpha
M-Ficolin
Database Links Reactome: R-HSA-166662
Reactome: R-HSA-166663
Reactome: R-HSA-2855086
Reactome: R-HSA-6798695
Cellular Localisation Secreted
Cell Membrane
Peripheral Membrane Protein
Extracellular Side
Found On The Monocyte And Granulocyte Surface
Alternative CLIA Names Ficolin-1 CLIA kit
Collagen/Fibrinogen Domain-Containing Protein 1 CLIA kit
Ficolin-A CLIA kit
Ficolin-Alpha CLIA kit
M-Ficolin CLIA kit
FCN1 CLIA kit
FCNM CLIA kit
Specificity This kit recognizes Human FCN1 in samples. No significant cross-reactivity or interference between Human FCN1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-97 92
EDTA plasma(n=8) 98-111 104
Cell culture media(n=8) 85-99 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 94.89 248.25 947.31 94.87 250.57 976.47
Standard deviation 10.68 29.47 104.01 8.07 27.79 104.29
CV (%) 11.26 11.87 10.98 8.51 11.09 10.68
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance