| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human FABP6 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
FABP6 |
| Gene ID: |
2172 |
| Uniprot ID: |
FABP6_HUMAN |
| Specificity: |
This kit recognizes Human FABP6 in samples. No significant cross-reactivity or interference between Human FABP6 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Isoform 1 is expressed in the jejunum, ileum, cecum and ascending colon intestine. Isoform 2 is xpressed in the gallbladder, duodenum, jejunum, ileum, cecum, ascending, transverse and descending colon, sigmoid colon and rectum. Isoform 2 is expressed in colorectal adenocarcinomas and their adjacent normal mucosa (at protein level). |
| Function | Binds to bile acids and is involved in enterohepatic bile acid metabolism. Required for efficient apical to basolateral transport of conjugated bile acids in ileal enterocytes. In vitro binds to bile acids in the order: deoxycholic acid > cholic acid > chenodeoxycholic acid and respective BA conjugation modifies affinities in the order taurine-conjugated > glycine-conjugated > unconjugated bile acids. Stimulates gastric acid and pepsinogen secretion. Isoform 2: Essential for the survival of colon cancer cells to bile acid-induced apoptosis. |
| Protein Name | GastrotropinGtFatty Acid-Binding Protein 6Ileal Lipid-Binding ProteinIlbpIntestinal 15 Kda ProteinI-15pIntestinal Bile Acid-Binding ProteinI-Babp |
| Database Links | Reactome: R-HSA-159418Reactome: R-HSA-163560Reactome: R-HSA-9623433 |
| Cellular Localisation | Isoform 1: CytoplasmMembranePeripheral Membrane ProteinCytoplasmic SideIsoform 2: CytoplasmLocalized Close To Nucleus On The Apical Side Of Both Normal And Neoplastic Cells |
| Alternative CLIA Names | Gastrotropin CLIA kitGt CLIA kitFatty Acid-Binding Protein 6 CLIA kitIleal Lipid-Binding Protein CLIA kitIlbp CLIA kitIntestinal 15 Kda Protein CLIA kitI-15p CLIA kitIntestinal Bile Acid-Binding Protein CLIA kitI-Babp CLIA kitFABP6 CLIA kitILBP CLIA kitILLBP CLIA kit |
| Specificity | This kit recognizes Human FABP6 in samples. No significant cross-reactivity or interference between Human FABP6 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-106 | 98 |
| EDTA plasma(n=8) | 95-108 | 101 |
| Cell culture media(n=8) | 89-99 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 96.45 | 225.80 | 693.13 | 101.19 | 234.01 | 705.45 |
| Standard deviation | 9.63 | 17.97 | 54.27 | 11.32 | 22.68 | 61.02 |
| CV (%) | 9.98 | 7.96 | 7.83 | 11.19 | 9.69 | 8.65 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
