| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This human EXT1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
EXT1 |
| Gene ID: |
2131 |
| Uniprot ID: |
EXT1_HUMAN |
| Specificity: |
This kit recognizes Human EXT1 in samples. No significant cross-reactivity or interference between Human EXT1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Ubiquitous. |
| Function | Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Appears to be a tumor suppressor. Required for the exosomal release of SDCBP, CD63 and syndecan. |
| Protein Name | Exostosin-1Glucuronosyl-N-Acetylglucosaminyl-Proteoglycan/N-Acetylglucosaminyl-Proteoglycan 4-Alpha-N-AcetylglucosaminyltransferaseMultiple Exostoses Protein 1Putative Tumor Suppressor Protein Ext1 |
| Database Links | Reactome: R-HSA-2022928Reactome: R-HSA-3656237Reactome: R-HSA-3656253 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Ii Membrane ProteinGolgi Apparatus MembraneGolgi ApparatusCis-Golgi Network MembraneThe Ext1/Ext2 Complex Is Localized In The Golgi Apparatus |
| Alternative CLIA Names | Exostosin-1 CLIA kitGlucuronosyl-N-Acetylglucosaminyl-Proteoglycan/N-Acetylglucosaminyl-Proteoglycan 4-Alpha-N-Acetylglucosaminyltransferase CLIA kitMultiple Exostoses Protein 1 CLIA kitPutative Tumor Suppressor Protein Ext1 CLIA kitEXT1 CLIA kit |
| Specificity | This kit recognizes Human EXT1 in samples. No significant cross-reactivity or interference between Human EXT1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 99-112 | 104 |
| EDTA plasma(n=8) | 96-106 | 101 |
| Cell culture media(n=8) | 96-110 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.53 | 1.42 | 4.28 | 0.49 | 1.38 | 4.10 |
| Standard deviation | 0.06 | 0.14 | 0.44 | 0.05 | 0.10 | 0.47 |
| CV (%) | 11.32 | 9.86 | 10.28 | 10.20 | 7.25 | 11.46 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
