| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This ESR1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
ESR1 |
| Gene ID: |
2099 |
| Uniprot ID: |
ESR1_HUMAN |
| Specificity: |
This kit recognizes Human ER Alpha in samples. No significant cross-reactivity or interference between Human ER Alpha and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated by cyclin A/CDK2 and CK1. Phosphorylation probably enhances transcriptional activity. Self-association induces phosphorylation. Dephosphorylation at Ser-118 by PPP5C inhibits its transactivation activity. Phosphorylated by LMTK3 in vitro. Glycosylated.contains N-acetylglucosamine, probably O-linked. Ubiquitinated.regulated by LATS1 via DCAF1 it leads to ESR1 proteasomal degradation. Deubiquitinated by OTUB1. Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization. Demethylated by JMJD6 at Arg-260. Palmitoylated (isoform 3). Not biotinylated (isoform 3). Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation, but not for signaling mediated by the nuclear hormone receptor. |
| Function | Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements.the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3: Involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor. Binds to ERE and inhibits isoform 1. |
| Protein Name | Estrogen ReceptorErEr-AlphaEstradiol ReceptorNuclear Receptor Subfamily 3 Group A Member 1 |
| Database Links | Reactome: R-HSA-1251985Reactome: R-HSA-1257604Reactome: R-HSA-2219530Reactome: R-HSA-383280Reactome: R-HSA-4090294Reactome: R-HSA-5689896Reactome: R-HSA-6811558Reactome: R-HSA-8866910Reactome: R-HSA-8931987Reactome: R-HSA-8939211Reactome: R-HSA-8939256Reactome: R-HSA-8939902Reactome: R-HSA-9009391Reactome: R-HSA-9018519 |
| Cellular Localisation | Isoform 1: NucleusCytoplasmCell MembranePeripheral Membrane ProteinCytoplasmic SideA Minor Fraction Is Associated With The Inner MembraneIsoform 3: NucleusSingle-Pass Type I Membrane ProteinAssociated With The Inner Membrane Via Palmitoylation (Probable)At Least A Subset Exists As A Transmembrane Protein With A N-Terminal Extracellular DomainNucleusGolgi ApparatusColocalizes With Zdhhc7 And Zdhhc21 In The Golgi Apparatus Where Most Probably Palmitoylation OccursAssociated With The Plasma Membrane When Palmitoylated |
| Alternative ELISA Names | Estrogen Receptor ELISA kitEr ELISA kitEr-Alpha ELISA kitEstradiol Receptor ELISA kitNuclear Receptor Subfamily 3 Group A Member 1 ELISA kitESR1 ELISA kitESR ELISA kitNR3A1 ELISA kit |
| Specificity | This kit recognizes Human ERα in samples. No significant cross-reactivity or interference between Human ERα and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-103 | 98 |
| EDTA plasma (n=8) | 85-98 | 90 |
| Cell culture media (n=8) | 92-106 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 192.09 | 465.77 | 1900.81 | 203.53 | 448.94 | 1773.53 |
| Standard deviation | 12.02 | 26.08 | 89.15 | 12.60 | 26.26 | 53.38 |
| CV (%) | 6.26 | 5.60 | 4.69 | 6.19 | 5.85 | 3.01 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
