| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.117ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This ERa Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Esr1 |
| Gene ID: |
24890 |
| Uniprot ID: |
ESR1_RAT |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Phosphorylated by cyclin A/CDK2 and CK1. Phosphorylation probably enhances transcriptional activity. Dephosphorylation at Ser-123 by PPP5C inhibits its transactivation activity. Phosphorylated by LMTK3 (in vitro). Ubiquitinated.regulated by LATS1 via DCAF1 it leads to ESR1 proteasomal degradation. Deubiquitinated by OTUB1. Palmitoylated at Cys-452 by ZDHHC7 and ZDHHC21. This modification is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation, but not for signaling mediated by the nuclear hormone receptor. Dimethylated by PRMT1 at Arg-265. The methylation may favor cytoplasmic localization. Demethylated by JMJD6 at Arg-265. |
| Function | Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements.the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. |
| Protein Name | Estrogen ReceptorErEr-AlphaEstradiol ReceptorNuclear Receptor Subfamily 3 Group A Member 1 |
| Database Links | Reactome: R-RNO-1251985Reactome: -RNO-1257604Reactome: -RNO-383280Reactome: -RNO-4090294Reactome: -RNO-5689896Reactome: -RNO-6811558Reactome: -RNO-8866910Reactome: -RNO-8931987Reactome: -RNO-8939211Reactome: -RNO-9009391Reactome: -RNO-9018519 |
| Cellular Localisation | NucleusCytoplasmGolgi ApparatusCell MembraneColocalizes With Zdhhc7 And Zdhhc21 In The Golgi Apparatus Where Most Probably Palmitoylation OccursAssociated With The Plasma Membrane When Palmitoylated |
| Alternative ELISA Names | Estrogen Receptor ELISA kitEr ELISA kitEr-Alpha ELISA kitEstradiol Receptor ELISA kitNuclear Receptor Subfamily 3 Group A Member 1 ELISA kitEsr1 ELISA kitEsr ELISA kitEstr ELISA kitNr3a1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
