| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human EPYC kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
EPYC |
| Gene ID: |
1833 |
| Uniprot ID: |
EPYC_HUMAN |
| Specificity: |
This kit recognizes Human EPYC in samples. No significant cross-reactivity or interference between Human EPYC and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Cartilage, ligament, and placenta. |
| Post Translational Modifications | The O-linked polysaccharides on Thr-60 and Ser-96 are probably the mucin type linked to GalNAc. There is one glycosaminoglycan chain, known to be dermatan sulfate, and it is probably the O-glycosylation at Ser-64. |
| Function | May have a role in bone formation and also in establishing the ordered structure of cartilage through matrix organization. |
| Protein Name | EpiphycanDermatan Sulfate Proteoglycan 3Proteoglycan-LbPg-LbSmall Chondroitin/Dermatan Sulfate Proteoglycan |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative CLIA Names | Epiphycan CLIA kitDermatan Sulfate Proteoglycan 3 CLIA kitProteoglycan-Lb CLIA kitPg-Lb CLIA kitSmall Chondroitin/Dermatan Sulfate Proteoglycan CLIA kitEPYC CLIA kitDSPG3 CLIA kitPGLB CLIA kitSLRR3B CLIA kit |
| Specificity | This kit recognizes Human EPYC in samples. No significant cross-reactivity or interference between Human EPYC and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-101 | 92 |
| EDTA plasma(n=8) | 98-114 | 104 |
| Cell culture media(n=8) | 84-96 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 109.35 | 296.53 | 741.01 | 104.43 | 289.51 | 712.34 |
| Standard deviation | 9.12 | 34.25 | 56.91 | 9.05 | 32.11 | 58.84 |
| CV (%) | 8.34 | 11.55 | 7.68 | 8.67 | 11.09 | 8.26 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
