| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human EFNA4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
EFNA4 |
| Gene ID: |
1945 |
| Uniprot ID: |
EFNA4_HUMAN |
| Specificity: |
This kit recognizes Human EFNA4 in samples. No significant cross-reactivity or interference between Human EFNA4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in the adult spleen, lymph node, prostate, ovary, small intestine, and colon, and in fetal heart, lung, liver and kidney. Also detected in hematopoietic cell lines. |
| Function | Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. May play a role in the interaction between activated B-lymphocytes and dendritic cells in tonsils. |
| Protein Name | Ephrin-A4Eph-Related Receptor Tyrosine Kinase Ligand 4Lerk-4 |
| Database Links | Reactome: R-HSA-2682334Reactome: R-HSA-3928663Reactome: R-HSA-3928665 |
| Cellular Localisation | Isoform 1: Cell MembraneLipid-AnchorGpi-AnchorIsoform 2: Secreted |
| Alternative CLIA Names | Ephrin-A4 CLIA kitEph-Related Receptor Tyrosine Kinase Ligand 4 CLIA kitLerk-4 CLIA kitEFNA4 CLIA kitEPLG4 CLIA kitLERK4 CLIA kit |
| Specificity | This kit recognizes Human EFNA4 in samples. No significant cross-reactivity or interference between Human EFNA4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-98 | 92 |
| EDTA plasma(n=8) | 99-115 | 106 |
| Cell culture media(n=8) | 99-115 | 106 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 204.15 | 467.51 | 1666.97 | 215.83 | 494.87 | 1636.15 |
| Standard deviation | 18.88 | 40.72 | 173.36 | 26.16 | 41.42 | 119.11 |
| CV (%) | 9.25 | 8.71 | 10.40 | 12.12 | 8.37 | 7.28 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
