| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human DRD2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
DRD2 |
| Gene ID: |
1813 |
| Uniprot ID: |
DRD2_HUMAN |
| Specificity: |
This kit recognizes Human D2R/DRD2 in samples. No significant cross-reactivity or interference between Human D2R/DRD2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Isoform 1: Expressed in the anterior pituitary gland. Isoform 2: Expressed in the anterior pituitary gland. |
| Post Translational Modifications | Palmitoylated. Palmitoylation which is required for proper localization to the plasma membrane and stability of the receptor could be carried on by ZDHHC4, ZDHHC3 and ZDHHC8. |
| Function | Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Positively regulates postnatal regression of retinal hyaloid vessels via suppression of VEGFR2/KDR activity, downstream of OPN5. |
| Protein Name | D(2 Dopamine ReceptorDopamine D2 Receptor |
| Database Links | Reactome: R-HSA-390651 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinGolgi Apparatus Membrane |
| Alternative CLIA Names | D(2 Dopamine Receptor CLIA kitDopamine D2 Receptor CLIA kitDRD2 CLIA kit |
| Specificity | This kit recognizes Human D2R/DRD2 in samples. No significant cross-reactivity or interference between Human D2R/DRD2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 98-111 | 103 |
| EDTA plasma(n=8) | 90-106 | 96 |
| Cell culture media(n=8) | 86-100 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 220.37 | 383.70 | 1849.86 | 228.19 | 396.74 | 1768.54 |
| Standard deviation | 20.34 | 36.53 | 204.41 | 24.12 | 46.78 | 188.17 |
| CV (%) | 9.23 | 9.52 | 11.05 | 10.57 | 11.79 | 10.64 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
