| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This human DNM2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
DNM2 |
| Gene ID: |
1785 |
| Uniprot ID: |
DYN2_HUMAN |
| Specificity: |
This kit recognizes Human DNM2 in samples. No significant cross-reactivity or interference between Human DNM2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Ubiquitously expressed. |
| Post Translational Modifications | Phosphorylation at Ser-764 by CDK1 is greatly increased upon mitotic entry. It regulates cytokinesis downstream of calcineurin, and does not affect clathrin-mediated endocytosis. Dephosphorylated by calcineurin/PP2. Phosphorylated on tyrosine residues after activation of SRC. |
| Function | Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis. Regulates maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane. |
| Protein Name | Dynamin-2 |
| Database Links | Reactome: R-HSA-166016Reactome: R-HSA-177504Reactome: R-HSA-190873Reactome: R-HSA-196025Reactome: R-HSA-203641Reactome: R-HSA-2132295Reactome: R-HSA-432720Reactome: R-HSA-432722Reactome: R-HSA-437239Reactome: R-HSA-8856828Reactome: R-HSA-9031628 |
| Cellular Localisation | CytoplasmCytoskeletonCell JunctionMembraneClathrin-Coated PitPostsynaptic DensitySynapseMidbodyCell ProjectionPhagocytic CupCytoplasmic VesiclePhagosome MembranePeripheral Membrane ProteinColocalizes With Cttn At The Basis Of Filopodia In Hippocampus Neuron Growth ZonesMicrotubule-AssociatedAlso Found In The Postsynaptic Density Of Neuronal CellsCo-Localizes With Pik3c3 And Rab5a To The Nascent Phagosome |
| Alternative CLIA Names | Dynamin-2 CLIA kitDNM2 CLIA kitDYN2 CLIA kit |
| Specificity | This kit recognizes Human DNM2 in samples. No significant cross-reactivity or interference between Human DNM2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 100-115 | 106 |
| EDTA plasma(n=8) | 88-100 | 93 |
| Cell culture media(n=8) | 87-101 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 265.72 | 679.15 | 2300.15 | 282.12 | 708.89 | 2278.93 |
| Standard deviation | 32.10 | 51.00 | 194.59 | 34.90 | 61.67 | 253.42 |
| CV (%) | 12.08 | 7.51 | 8.46 | 12.37 | 8.70 | 11.12 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
