| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This DNASE1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
DNASE1 |
| Gene ID: |
1773 |
| Uniprot ID: |
DNAS1_HUMAN |
| Specificity: |
This kit recognizes Human DNase-1 in samples. No significant cross-reactivity or interference between Human DNase-1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation. |
| Protein Name | Deoxyribonuclease-1Deoxyribonuclease IDnase IDornase Alfa |
| Cellular Localisation | SecretedZymogen GranuleNucleus EnvelopeSecretory ProteinStored In Zymogen Granules And Found In The Nuclear Envelope |
| Alternative ELISA Names | Deoxyribonuclease-1 ELISA kitDeoxyribonuclease I ELISA kitDnase I ELISA kitDornase Alfa ELISA kitDNASE1 ELISA kitDNL1 ELISA kitDRNI ELISA kit |
| Specificity | This kit recognizes Human DNase-Ⅰ in samples. No significant cross-reactivity or interference between Human DNase-Ⅰ and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-103 | 95 |
| EDTA plasma (n=8) | 93-108 | 100 |
| Cell culture media (n=8) | 85-99 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.53 | 1.21 | 3.89 | 0.52 | 1.24 | 3.97 |
| Standard deviation | 0.04 | 0.07 | 0.15 | 0.03 | 0.06 | 0.16 |
| CV (%) | 7.55 | 5.79 | 3.86 | 5.77 | 4.84 | 4.03 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
