| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human DLK1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
DLK1 |
| Gene ID: |
8788 |
| Uniprot ID: |
DLK1_HUMAN |
| Specificity: |
This kit recognizes Human DLK-1 in samples. No significant cross-reactivity or interference between Human DLK-1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. |
| Post Translational Modifications | N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. |
| Function | May have a role in neuroendocrine differentiation. |
| Protein Name | Protein Delta Homolog 1Dlk-1Pg2 Cleaved Into - Fetal Antigen 1Fa1 |
| Database Links | Reactome: R-HSA-2122948 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane ProteinCytoplasm |
| Alternative CLIA Names | Protein Delta Homolog 1 CLIA kitDlk-1 CLIA kitPg2 Cleaved Into - Fetal Antigen 1 CLIA kitFa1 CLIA kitDLK1 CLIA kitDLK CLIA kit |
| Specificity | This kit recognizes Human DLK-1 in samples. No significant cross-reactivity or interference between Human DLK-1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 84-98 | 90 |
| EDTA plasma(n=8) | 95-109 | 102 |
| Cell culture media(n=8) | 89-101 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 95.75 | 200.79 | 694.27 | 96.69 | 204.04 | 671.36 |
| Standard deviation | 9.52 | 22.95 | 65.89 | 8.67 | 22.79 | 53.71 |
| CV (%) | 9.94 | 11.43 | 9.49 | 8.97 | 11.17 | 8.00 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
