| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human DKK4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
DKK4 |
| Gene ID: |
27121 |
| Uniprot ID: |
DKK4_HUMAN |
| Specificity: |
This kit recognizes Human DKK4 in samples. No significant cross-reactivity or interference between Human DKK4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in cerebellum, T-cells, esophagus and lung. |
| Post Translational Modifications | Appears to be not glycosylated. Can be proteolytically processed by a furin-like protease. |
| Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. |
| Protein Name | Dickkopf-Related Protein 4Dickkopf-4Dkk-4Hdkk-4 Cleaved Into - Dickkopf-Related Protein 4 Short Form |
| Database Links | Reactome: R-HSA-201681Reactome: R-HSA-3772470Reactome: R-HSA-5339717 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Dickkopf-Related Protein 4 CLIA kitDickkopf-4 CLIA kitDkk-4 CLIA kitHdkk-4 Cleaved Into - Dickkopf-Related Protein 4 Short Form CLIA kitDKK4 CLIA kit |
| Specificity | This kit recognizes Human DKK4 in samples. No significant cross-reactivity or interference between Human DKK4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-107 | 100 |
| EDTA plasma(n=8) | 100-116 | 107 |
| Cell culture media(n=8) | 97-112 | 104 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 101.24 | 319.22 | 869.44 | 102.31 | 299.60 | 812.03 |
| Standard deviation | 10.74 | 37.09 | 81.55 | 12.56 | 32.99 | 57.98 |
| CV (%) | 10.61 | 11.62 | 9.38 | 12.28 | 11.01 | 7.14 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
