• Human DEFa6 (Defensin Alpha 6, Paneth Cell Specific) Sandwich ELISA Kit (STJE0008398)

Human DEFa6 (Defensin Alpha 6, Paneth Cell Specific) Sandwich ELISA Kit (STJE0008398)

SKU:
STJE0008398

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 1.07ng/mL
Detection Limit: 2.34-150ng/mL
Short Description: This DEFa6 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: DEFA6
Gene ID: 1671
Uniprot ID: DEF6_HUMAN
Sample Type: serum, plasma, tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity Expressed in Paneth cells of the small intestine (at protein level).
Post Translational Modifications Proteolytically cleaved by trypsin at Arg-68.the propeptide is stored in the tissue of the small intestine and the mature peptide is found in the luminal fluid.cleavage may occur during or after release into the lumen. The N-terminal propeptide region suppresses self-assembly and renders DEFA6 propeptide unable to agglutinate bacteria and protect human epithelial cells from bacterial invasion. Under reducing conditions, naturally present in the gut owing to the low redox potential or enzymatically generated by the thioredoxin system, the disulfide bridges are opened leading to a conformational change of DEF6, thereby changing its antimicrobial spectrum. The reduced form exhibits inhibitory activity against anaerobic bacteria, in contrast to the minimal antimicrobial activity of the disulfide-linked oxidized form. The formation of higher-order nanonets and bacterial entrapment is independent of the redox state.
Function Host-defense peptide that contributes to intestinal innate immunity and mediates homeostasis at mucosal surfaces by forming higher-order oligomers that capture bacteria and prevent microbial invasion of the epithelium. After binding to bacterial surface proteins, undergoes ordered self-assembly to form fibril-like nanonets that surround and entangle bacteria and thereby prevent bacterial invasion across the epithelial barrier. Entangles and agglutinates Gram-negative bacteria, such as E.coli, S.typhimurium and Y.enterocolitica, and Gram-positive bacteria such as L.monocytogenes, thereby protecting the intestine against invasion by enteric bacterial pathogens. Blocks adhesion of C.albicans to intestinal epithelial cells and thereby suppresses fungal invasion of epithelial cells and biofilm formation. Under reducing conditions and in an acidic environment similar to the intestinal milieu, exhibits inhibitory activity against anaerobic bacteria such as B.adolescentis, L.acidophilus and B.breve, as well as B.longum and S.thermophilus, possibly by leading to alterations in bacterial cell envelope structures. The disulfide-linked oxidized form exhibits negligible antimicrobial activity against Gram-negative and Gram-positive bacteria, as compared to the enteric defensin DEFA5.
Protein Name Defensin-6
Defensin - Alpha 6
Database Links Reactome: R-HSA-1461973
Reactome: R-HSA-1462054
Cellular Localisation Secreted
Cytoplasmic Vesicle
Secretory Vesicle
Stored As Propeptide In Secretory Granules Of Small Intestinal Paneth Cells And Found In The Ileum Lumen As Mature Peptide
Alternative ELISA Names Defensin-6 ELISA kit
Defensin - Alpha 6 ELISA kit
DEFA6 ELISA kit
DEF6 ELISA kit
output

Information sourced from Uniprot.org

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