| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.061ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This DDH2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
AKR1C2 |
| Gene ID: |
1646 |
| Uniprot ID: |
AK1C2_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Expressed in fetal testes. Expressed in fetal and adult adrenal glands. |
| Post Translational Modifications | |
| Function | Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH. Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens. Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha-DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Also specifically able to produce 17beta-hydroxy-5alpha-androstan-3-one/5alphaDHT. May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate. Displays affinity for bile acids. |
| Protein Name | Aldo-Keto Reductase Family 1 Member C2 3-Alpha-Hsd3Chlordecone Reductase Homolog HakrdDihydrodiol Dehydrogenase 2Dd-2Dd2Dihydrodiol Dehydrogenase/Bile Acid-Binding ProteinDd/BabpType Iii 3-Alpha-Hydroxysteroid Dehydrogenase |
| Database Links | Reactome: R-HSA-193368Reactome: R-HSA-193775Reactome: R-HSA-193807 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative ELISA Names | Aldo-Keto Reductase Family 1 Member C2 ELISA kit3-Alpha-Hsd3 ELISA kitChlordecone Reductase Homolog Hakrd ELISA kitDihydrodiol Dehydrogenase 2 ELISA kitDd-2 ELISA kitDd2 ELISA kitDihydrodiol Dehydrogenase/Bile Acid-Binding Protein ELISA kitDd/Babp ELISA kitType Iii 3-Alpha-Hydroxysteroid Dehydrogenase ELISA kitAKR1C2 ELISA kitDDH2 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
