| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human CYP24A1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CYP24A1 |
| Gene ID: |
1591 |
| Uniprot ID: |
CP24A_HUMAN |
| Specificity: |
This kit recognizes Human CYP24A1 in samples. No significant cross-reactivity or interference between Human CYP24A1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D(3)) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid. With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product. Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin. |
| Protein Name | 1 -25-Dihydroxyvitamin D(3 24-Hydroxylase - Mitochondrial24-OhaseVitamin D(3 24-HydroxylaseCytochrome P450 24a1Cytochrome P450-Cc24 |
| Database Links | Reactome: R-HSA-196791Reactome: R-HSA-211916Reactome: R-HSA-5579010 |
| Cellular Localisation | Mitochondrion |
| Alternative CLIA Names | 1 -25-Dihydroxyvitamin D(3 24-Hydroxylase - Mitochondrial CLIA kit24-Ohase CLIA kitVitamin D(3 24-Hydroxylase CLIA kitCytochrome P450 24a1 CLIA kitCytochrome P450-Cc24 CLIA kitCYP24A1 CLIA kitCYP24 CLIA kit |
| Specificity | This kit recognizes Human CYP24A1 in samples. No significant cross-reactivity or interference between Human CYP24A1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-109 | 100 |
| EDTA plasma(n=8) | 89-101 | 94 |
| Cell culture media(n=8) | 98-111 | 105 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 105.39 | 206.82 | 902.96 | 96.54 | 197.55 | 857.08 |
| Standard deviation | 11.45 | 15.02 | 84.61 | 12.38 | 16.38 | 64.45 |
| CV (%) | 10.86 | 7.26 | 9.37 | 12.82 | 8.29 | 7.52 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
