• Human CYP11B1 (Cytochrome P450 11B1) Sandwich ELISA Kit (STJE0008302)

Human CYP11B1 (Cytochrome P450 11B1) Sandwich ELISA Kit (STJE0008302)

SKU:
STJE0008302

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.074ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This CYP11B1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: CYP11B1
Gene ID: 1584
Uniprot ID: C11B1_HUMAN
Sample Type: serum, plasma, tissue homogenates or other biological fluids.
Tissue Specificity Expressed in the zona fasciculata/reticularis of the adrenal cortex.
Post Translational Modifications
Function A cytochrome P450 monooxygenase involved in the biosynthesis of adrenal corticoids. Catalyzes a variety of reactions that are essential for many species, including detoxification, defense, and the formation of endogenous chemicals like steroid hormones. Steroid 11beta, 18- and 19-hydroxylase with preferred regioselectivity at 11beta, then 18, and lastly 19. Catalyzes the hydroxylation of 11-deoxycortisol and 11-deoxycorticosterone (21-hydroxyprogesterone) at 11beta position, yielding cortisol or corticosterone, respectively, but cannot produce aldosterone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate for hydroxylation and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Due to its lack of 18-oxidation activity, it is incapable of generating aldosterone. Could also be involved in the androgen metabolic pathway (Probable).
Protein Name Cytochrome P450 11b1 - Mitochondrial
Cyp11b1
Cypxib1
Cytochrome P-450c11
Cytochrome P450c11
Steroid 11-Beta-Hydroxylase - Cyp11b1
Database Links Reactome: R-HSA-194002
Reactome: R-HSA-211976
Reactome: R-HSA-5579017
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Alternative ELISA Names Cytochrome P450 11b1 - Mitochondrial ELISA kit
Cyp11b1 ELISA kit
Cypxib1 ELISA kit
Cytochrome P-450c11 ELISA kit
Cytochrome P450c11 ELISA kit
Steroid 11-Beta-Hydroxylase - Cyp11b1 ELISA kit
CYP11B1 ELISA kit
S11BH ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance