| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This CXCR4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CXCR4 |
| Gene ID: |
7852 |
| Uniprot ID: |
CXCR4_HUMAN |
| Specificity: |
This kit recognizes Human CXCR4 in samples. No significant cross-reactivity or interference between Human CXCR4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated on agonist stimulation. Rapidly phosphorylated on serine and threonine residues in the C-terminal. Phosphorylation at Ser-324 and Ser-325 leads to recruitment of ITCH, ubiquitination and protein degradation. Ubiquitinated after ligand binding, leading to its degradation. Ubiquitinated by ITCH at the cell membrane on agonist stimulation. The ubiquitin-dependent mechanism, endosomal sorting complex required for transport (ESCRT), then targets CXCR4 for lysosomal degradation. This process is dependent also on prior Ser-/Thr-phosphorylation in the C-terminal of CXCR4. Also binding of ARRB1 to STAM negatively regulates CXCR4 sorting to lysosomes though modulating ubiquitination of SFR5S. Sulfation on Tyr-21 is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization. Tyr-7 and Tyr-12 are sulfated in a sequential manner after Tyr-21 is almost fully sulfated, with the binding affinity for CXCL12/SDF-1alpha increasing with the number of sulfotyrosines present. Sulfotyrosines Tyr-7 and Tyr-12 occupy clefts on opposing CXCL12 subunits, thus bridging the CXCL12 dimer interface and promoting CXCL12 dimerization. O- and N-glycosylated. Asn-11 is the principal site of N-glycosylation. There appears to be very little or no glycosylation on Asn-176. N-glycosylation masks coreceptor function in both X4 and R5 laboratory-adapted and primary HIV-1 strains through inhibiting interaction with their Env glycoproteins. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity. |
| Function | Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Involved in the AKT signaling cascade. Plays a role in regulation of cell migration, e.g. during wound healing. Acts as a receptor for extracellular ubiquitin.leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) for human immunodeficiency virus-1/HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. |
| Protein Name | C-X-C Chemokine Receptor Type 4Cxc-R4Cxcr-4Fb22FusinHm89Lcr1Leukocyte-Derived Seven Transmembrane Domain ReceptorLestrLipopolysaccharide-Associated Protein 3Lap-3Lps-Associated Protein 3NpyrlStromal Cell-Derived Factor 1 ReceptorSdf-1 ReceptorCd Antigen Cd184 |
| Database Links | Reactome: R-HSA-173107Reactome: R-HSA-376176Reactome: R-HSA-380108Reactome: R-HSA-418594Reactome: R-HSA-9823730Reactome: R-HSA-9827857 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell JunctionEarly EndosomeLate EndosomeLysosomeIn Unstimulated CellsDiffuse Pattern On Plasma MembraneOn Agonist StimulationColocalizes With Itch At The Plasma Membrane Where It Becomes UbiquitinatedIn The Presence Of AntigenDistributes To The Immunological Synapse Forming At The T-Cell-Apc Contact AreaWhere It Localizes At The Peripheral And Distal Supramolecular Activation Cluster (Smac) |
| Alternative ELISA Names | C-X-C Chemokine Receptor Type 4 ELISA kitCxc-R4 ELISA kitCxcr-4 ELISA kitFb22 ELISA kitFusin ELISA kitHm89 ELISA kitLcr1 ELISA kitLeukocyte-Derived Seven Transmembrane Domain Receptor ELISA kitLestr ELISA kitLipopolysaccharide-Associated Protein 3 ELISA kitLap-3 ELISA kitLps-Associated Protein 3 ELISA kitNpyrl ELISA kitStromal Cell-Derived Factor 1 Receptor ELISA kitSdf-1 Receptor ELISA kitCd Antigen Cd184 ELISA kitCXCR4 ELISA kit |
| Specificity | This kit recognizes Human CXCR4 in samples. No significant cross-reactivity or interference between Human CXCR4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-108 | 100 |
| EDTA plasma (n=8) | 85-101 | 92 |
| Cell culture media (n=8) | 85-95 | 90 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 267.75 | 731.87 | 1676.97 | 256.77 | 723.66 | 1746.63 |
| Standard deviation | 15.88 | 32.71 | 84.18 | 17.08 | 39.51 | 85.76 |
| CV (%) | 5.93 | 4.47 | 5.02 | 6.65 | 5.46 | 4.91 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
