| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
27.2pg/mL |
| Detection Limit: |
78.1-5000pg/mL |
| Short Description: |
This CXCR4 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Cxcr4 |
| Gene ID: |
12767 |
| Uniprot ID: |
CXCR4_MOUSE |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Lymphocytes, macrophages, neutrophils, microglial cells and astrocytes. Found in spleen, thymus, bone marrow, lymph nodes and, at lower levels in brain, small intestine, stomach and kidney. CXCR4-A is predominant in all tissues tested. During embryonic development, high levels are detected in the endothelium of developing blood vessels and in many regions of the developing brain including the olfactory epithelium, olfactory bulb, hippocampus, cerebellum and spinal cord. |
| Post Translational Modifications | Phosphorylated on agonist stimulation. Rapidly phosphorylated on serine and threonine residues in the C-terminal. Phosphorylation at Ser-331 and Ser-332 leads to recruitment of ITCH, ubiquitination and protein degradation. Ubiquitinated after ligand binding, leading to its degradation. Ubiquitinated by ITCH at the cell membrane on agonist stimulation. The ubiquitin-dependent mechanism, endosomal sorting complex required for transport (ESCRT), then targets CXCR4 for lysosomal degradation. This process is dependent also on prior Ser-/Thr-phosphorylation in the C-terminal of CXCR4. Also binding of ARRB1 to STAM negatively regulates CXCR4 sorting to lysosomes though modulating ubiquitination of SFR5S. Sulfation is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization. O- and N-glycosylated. N-glycosylation can mask coreceptor function. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity. |
| Function | Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Involved in the AKT signaling cascade. Plays a role in regulation of cell migration, e.g. during wound healing. Acts as a receptor for extracellular ubiquitin.leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. |
| Protein Name | C-X-C Chemokine Receptor Type 4Cxc-R4Cxcr-4FusinLeukocyte-Derived Seven Transmembrane Domain ReceptorLestrPre-B-Cell-Derived Chemokine ReceptorPb-CkrStromal Cell-Derived Factor 1 ReceptorSdf-1 ReceptorCd Antigen Cd184 |
| Database Links | Reactome: R-MMU-376176Reactome: -MMU-380108Reactome: -MMU-418594 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell JunctionEarly EndosomeLate EndosomeLysosomeIn Unstimulated CellsDiffuse Pattern On Plasma MembraneOn Agonist StimulationColocalizes With Itch At The Plasma Membrane Where It Becomes UbiquitinatedIn The Presence Of AntigenDistributes To The Immunological Synapse Forming At The T-Cell-Apc Contact AreaWhere It Localizes At The Peripheral And Distal Supramolecular Activation Cluster (Smac) |
| Alternative ELISA Names | C-X-C Chemokine Receptor Type 4 ELISA kitCxc-R4 ELISA kitCxcr-4 ELISA kitFusin ELISA kitLeukocyte-Derived Seven Transmembrane Domain Receptor ELISA kitLestr ELISA kitPre-B-Cell-Derived Chemokine Receptor ELISA kitPb-Ckr ELISA kitStromal Cell-Derived Factor 1 Receptor ELISA kitSdf-1 Receptor ELISA kitCd Antigen Cd184 ELISA kitCxcr4 ELISA kitCmkar4 ELISA kitLestr ELISA kitSdf1r ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
