| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This human CXCL16 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CXCL16 |
| Gene ID: |
58191 |
| Uniprot ID: |
CXL16_HUMAN |
| Specificity: |
This kit recognizes Human GRO Alpha/CXCL1 in samples. No significant cross-reactivity or interference between Human GRO Alpha/CXCL1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in T-cell areas. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow. |
| Post Translational Modifications | Glycosylated. |
| Function | Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis. Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. |
| Protein Name | C-X-C Motif Chemokine 16Scavenger Receptor For Phosphatidylserine And Oxidized Low Density LipoproteinSr-PsoxSmall-Inducible Cytokine B16Transmembrane Chemokine Cxcl16 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-418594 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinSecretedAlso Exists As A Soluble Form |
| Alternative CLIA Names | C-X-C Motif Chemokine 16 CLIA kitScavenger Receptor For Phosphatidylserine And Oxidized Low Density Lipoprotein CLIA kitSr-Psox CLIA kitSmall-Inducible Cytokine B16 CLIA kitTransmembrane Chemokine Cxcl16 CLIA kitCXCL16 CLIA kitSCYB16 CLIA kitSRPSOX CLIA kitUNQ2759 CLIA kitPRO6714 CLIA kit |
| Specificity | This kit recognizes Human GROα/CXCL1 in samples. No significant cross-reactivity or interference between Human GROα/CXCL1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-106 | 99 |
| EDTA plasma(n=8) | 100-116 | 108 |
| Cell culture media(n=8) | 99-116 | 107 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 38.54 | 104.64 | 305.18 | 35.77 | 105.44 | 307.53 |
| Standard deviation | 4.18 | 11.18 | 34.82 | 3.19 | 9.04 | 29.40 |
| CV (%) | 10.85 | 10.68 | 11.41 | 8.92 | 8.57 | 9.56 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
