| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This human CXCL13 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CXCL13 |
| Gene ID: |
10563 |
| Uniprot ID: |
CXL13_HUMAN |
| Specificity: |
This kit recognizes Human BLC in samples. No significant cross-reactivity or interference between Human BLC and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen. |
| Function | Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5. |
| Protein Name | C-X-C Motif Chemokine 13AngieB Cell-Attracting Chemokine 1Bca-1B Lymphocyte ChemoattractantCxc Chemokine BlcSmall-Inducible Cytokine B13 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-418594 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | C-X-C Motif Chemokine 13 CLIA kitAngie CLIA kitB Cell-Attracting Chemokine 1 CLIA kitBca-1 CLIA kitB Lymphocyte Chemoattractant CLIA kitCxc Chemokine Blc CLIA kitSmall-Inducible Cytokine B13 CLIA kitCXCL13 CLIA kitBCA1 CLIA kitBLC CLIA kitSCYB13 CLIA kit |
| Specificity | This kit recognizes Human BLC in samples. No significant cross-reactivity or interference between Human BLC and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-107 | 99 |
| EDTA plasma(n=8) | 102-116 | 108 |
| Cell culture media(n=8) | 90-104 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 38.34 | 87.37 | 281.65 | 40.90 | 89.83 | 269.23 |
| Standard deviation | 4.57 | 7.35 | 24.48 | 4.80 | 6.53 | 21.75 |
| CV (%) | 11.92 | 8.41 | 8.69 | 11.74 | 7.27 | 8.08 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
