Mouse Cxcl13 (C-X-C motif chemokine 13) Sandwich ELISA (STJE0004328)

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STJE0004328

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This Cxcl13 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Cxcl13
Gene ID: 55985
Uniprot ID: CXL13_MOUSE
Specificity: This kit recognizes Mouse BLC in samples. No significant cross-reactivity or interference between Mouse BLC and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Strongly chemotactic for B-lymphocytes, weakly for spleen monocytes and macrophages but no chemotactic activity for granulocytes. Binds to BLR1/CXCR5. May play a role in directing the migration of B-lymphocytes to follicles in secondary lymphoid organs.
Protein Name C-X-C Motif Chemokine 13
B Lymphocyte Chemoattractant
Cxc Chemokine Blc
Small-Inducible Cytokine B13
Database Links Reactome: R-MMU-380108
Reactome: -MMU-418594
Cellular Localisation Secreted
Alternative ELISA Names C-X-C Motif Chemokine 13 ELISA kit
B Lymphocyte Chemoattractant ELISA kit
Cxc Chemokine Blc ELISA kit
Small-Inducible Cytokine B13 ELISA kit
Cxcl13 ELISA kit
Blc ELISA kit
Scyb13 ELISA kit
Specificity This kit recognizes Mouse BLC in samples. No significant cross-reactivity or interference between Mouse BLC and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-102 93
EDTA plasma (n=8) 91-103 96
Cell culture media (n=8) 89-100 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 51.22 89.77 396.03 48.37 85.44 408.04
Standard deviation 3.45 4.27 15.52 3.08 4.21 14.77
CV (%) 6.74 4.76 3.92 6.37 4.93 3.62
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance