| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.67ng/mL |
| Detection Limit: |
1.56-100ng/mL |
| Short Description: |
This cPLA2a Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
PLA2G4A |
| Gene ID: |
5321 |
| Uniprot ID: |
PA24A_HUMAN |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | Expressed in various cells and tissues such as macrophages, neutrophils, fibroblasts and lung endothelium. Expressed in platelets (at protein level). |
| Post Translational Modifications | Phosphorylated at both Ser-505 and Ser-727 in response to mitogenic stimuli. |
| Function | Has primarily calcium-dependent phospholipase and lysophospholipase activities, with a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. Plays an important role in embryo implantation and parturition through its ability to trigger prostanoid production. Preferentially hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Selectively hydrolyzes sn-2 arachidonoyl group from membrane phospholipids, providing the precursor for eicosanoid biosynthesis via the cyclooxygenase pathway. In an alternative pathway of eicosanoid biosynthesis, hydrolyzes sn-2 fatty acyl chain of eicosanoid lysophopholipids to release free bioactive eicosanoids. Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 position of phospholipids (phospholipase A1 activity) only if an ether linkage rather than an ester linkage is present at the sn-2 position. This hydrolysis is not stereospecific. Has calcium-independent phospholipase A2 and lysophospholipase activities in the presence of phosphoinositides. Has O-acyltransferase activity. Catalyzes the transfer of fatty acyl chains from phospholipids to a primary hydroxyl group of glycerol (sn-1 or sn-3), potentially contributing to monoacylglycerol synthesis. |
| Protein Name | Cytosolic Phospholipase A2Cpla2Phospholipase A2 Group Iva Includes - Phospholipase A2Phosphatidylcholine 2-Acylhydrolase - Lysophospholipase |
| Database Links | Reactome: R-HSA-111995Reactome: R-HSA-1482788Reactome: R-HSA-1482798Reactome: R-HSA-1482801Reactome: R-HSA-1482839Reactome: R-HSA-1482922Reactome: R-HSA-1482925Reactome: R-HSA-1483115Reactome: R-HSA-1483166Reactome: R-HSA-2142753Reactome: R-HSA-418592Reactome: R-HSA-432142Reactome: R-HSA-6811436 |
| Cellular Localisation | CytoplasmGolgi Apparatus MembraneNucleus EnvelopeTranslocates To Intracellular Membranes In A Calcium-Dependent Way |
| Alternative ELISA Names | Cytosolic Phospholipase A2 ELISA kitCpla2 ELISA kitPhospholipase A2 Group Iva Includes - Phospholipase A2 ELISA kitPhosphatidylcholine 2-Acylhydrolase - Lysophospholipase ELISA kitPLA2G4A ELISA kitCPLA2 ELISA kitPLA2G4 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
