• Human COL9A3 (Collagen alpha-3 (IX) chain) CLIA Kit (STJC0000458)

Human COL9A3 (Collagen alpha-3 (IX) chain) CLIA Kit (STJC0000458)

SKU:
STJC0000458

£238.50 - £477.50
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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This human COL9A3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: COL9A3
Gene ID: 1299
Uniprot ID: CO9A3_HUMAN
Specificity: This kit recognizes Human COL9 Alpha 3 in samples. No significant cross-reactivity or interference between Human COL9 Alpha 3 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Covalently linked to the telopeptides of type II collagen by lysine-derived cross-links. Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Function Structural component of hyaline cartilage and vitreous of the eye.
Protein Name Collagen Alpha-3(Ix Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1650814
Reactome: R-HSA-186797
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-3000178
Reactome: R-HSA-419037
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative CLIA Names Collagen Alpha-3(Ix Chain CLIA kit
COL9A3 CLIA kit
Specificity This kit recognizes Human COL9α3 in samples. No significant cross-reactivity or interference between Human COL9α3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-104 95
EDTA plasma(n=8) 89-104 96
Cell culture media(n=8) 91-103 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.51 0.97 4.14 0.53 1.06 4.06
Standard deviation 0.06 0.11 0.35 0.07 0.09 0.43
CV (%) 11.76 11.34 8.45 13.21 8.49 10.59
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance