| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This CLEC4E Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CLEC4E |
| Gene ID: |
26253 |
| Uniprot ID: |
CLC4E_HUMAN |
| Specificity: |
This kit recognizes Human CLEC4E in samples. No significant cross-reactivity or interference between Human CLEC4E and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Calcium-dependent lectin that acts as a pattern recognition receptor (PRR) of the innate immune system: recognizes damage-associated molecular patterns (DAMPs) of abnormal self and pathogen-associated molecular patterns (PAMPs) of bacteria and fungi. The PAMPs notably include mycobacterial trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid with potent adjuvant immunomodulatory functions. Interacts with signaling adapter Fc receptor gamma chain/FCER1G to form a functional complex in myeloid cells. Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes. Also recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia and mediates macrophage activation. Through recognition of DAMPs released upon nonhomeostatic cell death, enables immune sensing of damaged self and promotes inflammatory cell infiltration into the damaged tissue. |
| Protein Name | C-Type Lectin Domain Family 4 Member EC-Type Lectin Superfamily Member 9Macrophage-Inducible C-Type LectinMincle |
| Database Links | Reactome: R-HSA-5621480 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinCell ProjectionPhagocytic Cup |
| Alternative ELISA Names | C-Type Lectin Domain Family 4 Member E ELISA kitC-Type Lectin Superfamily Member 9 ELISA kitMacrophage-Inducible C-Type Lectin ELISA kitMincle ELISA kitCLEC4E ELISA kitCLECSF9 ELISA kitMINCLE ELISA kitUNQ218 ELISA kitPRO244 ELISA kit |
| Specificity | This kit recognizes Human CLEC4E in samples. No significant cross-reactivity or interference between Human CLEC4E and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-104 | 98 |
| EDTA plasma (n=8) | 90-102 | 96 |
| Cell culture media (n=8) | 93-105 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 244.41 | 742.23 | 2143.60 | 233.43 | 733.88 | 2131.05 |
| Standard deviation | 12.34 | 30.21 | 99.25 | 15.69 | 33.39 | 78.64 |
| CV (%) | 5.05 | 4.07 | 4.63 | 6.72 | 4.55 | 3.69 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
