| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human CLDN4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CLDN4 |
| Gene ID: |
1364 |
| Uniprot ID: |
CLD4_HUMAN |
| Specificity: |
This kit recognizes Human CLDN4 in samples. No significant cross-reactivity or interference between Human CLDN4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated. Phosphorylation by EPHA2 is stimulated by EFNA1 and alters interaction with TJP1. |
| Function | Channel-forming tight junction protein that mediates paracellular chloride transport in the kidney. Plays a critical role in the paracellular reabsorption of filtered chloride in the kidney collecting ducts. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. |
| Protein Name | Claudin-4Clostridium Perfringens Enterotoxin ReceptorCpe-RCpe-ReceptorWilliams-Beuren Syndrome Chromosomal Region 8 Protein |
| Database Links | Reactome: R-HSA-420029 |
| Cellular Localisation | Cell JunctionTight JunctionCell MembraneMulti-Pass Membrane ProteinCldn4 Is Required For Tight Junction Localization In The Kidney |
| Alternative CLIA Names | Claudin-4 CLIA kitClostridium Perfringens Enterotoxin Receptor CLIA kitCpe-R CLIA kitCpe-Receptor CLIA kitWilliams-Beuren Syndrome Chromosomal Region 8 Protein CLIA kitCLDN4 CLIA kitCPER CLIA kitCPETR1 CLIA kitWBSCR8 CLIA kit |
| Specificity | This kit recognizes Human CLDN4 in samples. No significant cross-reactivity or interference between Human CLDN4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-105 | 100 |
| EDTA plasma(n=8) | 98-110 | 104 |
| Cell culture media(n=8) | 85-99 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 104.91 | 257.13 | 941.04 | 102.07 | 258.00 | 1013.35 |
| Standard deviation | 11.98 | 26.69 | 89.59 | 8.52 | 25.39 | 89.99 |
| CV (%) | 11.42 | 10.38 | 9.52 | 8.35 | 9.84 | 8.88 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
