• Human CLDN4 (Claudin-4) Sandwich ELISA (STJE0002493)

Human CLDN4 (Claudin-4) Sandwich ELISA (STJE0002493)

SKU:
STJE0002493

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This CLDN4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CLDN4
Gene ID: 1364
Uniprot ID: CLD4_HUMAN
Specificity: This kit recognizes Human CLDN4 in samples. No significant cross-reactivity or interference between Human CLDN4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Channel-forming tight junction protein that mediates paracellular chloride transport in the kidney. Plays a critical role in the paracellular reabsorption of filtered chloride in the kidney collecting ducts. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Protein Name Claudin-4
Clostridium Perfringens Enterotoxin Receptor
Cpe-R
Cpe-Receptor
Williams-Beuren Syndrome Chromosomal Region 8 Protein
Database Links Reactome: R-HSA-420029
Cellular Localisation Cell Junction
Tight Junction
Cell Membrane
Multi-Pass Membrane Protein
Cldn4 Is Required For Tight Junction Localization In The Kidney
Alternative ELISA Names Claudin-4 ELISA kit
Clostridium Perfringens Enterotoxin Receptor ELISA kit
Cpe-R ELISA kit
Cpe-Receptor ELISA kit
Williams-Beuren Syndrome Chromosomal Region 8 Protein ELISA kit
CLDN4 ELISA kit
CPER ELISA kit
CPETR1 ELISA kit
WBSCR8 ELISA kit
Specificity This kit recognizes Human CLDN4 in samples. No significant cross-reactivity or interference between Human CLDN4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-108 102
EDTA plasma (n=8) 86-100 92
Cell culture media (n=8) 87-102 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.51 1.28 3.76 0.55 1.30 4.07
Standard deviation 0.03 0.06 0.13 0.03 0.07 0.15
CV (%) 5.88 4.69 3.46 5.45 5.38 3.69
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance