• Human CLDN3 (Claudin-3) Sandwich ELISA (STJE0002458)

Human CLDN3 (Claudin-3) Sandwich ELISA (STJE0002458)

SKU:
STJE0002458

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.19ng/mL
Detection Limit: 0.31~20ng/mL
Short Description: This CLDN3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CLDN3
Gene ID: 1365
Uniprot ID: CLD3_HUMAN
Specificity: This kit recognizes Human CLDN3 in samples. No significant cross-reactivity or interference between Human CLDN3 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Claudin-3
Clostridium Perfringens Enterotoxin Receptor 2
Cpe-R 2
Cpe-Receptor 2
Rat Ventral Prostate.1 Protein Homolog
Hrvp1
Database Links Reactome: R-HSA-420029
Cellular Localisation Cell Junction
Tight Junction
Cell Membrane
Multi-Pass Membrane Protein
Alternative ELISA Names Claudin-3 ELISA kit
Clostridium Perfringens Enterotoxin Receptor 2 ELISA kit
Cpe-R 2 ELISA kit
Cpe-Receptor 2 ELISA kit
Rat Ventral Prostate.1 Protein Homolog ELISA kit
Hrvp1 ELISA kit
CLDN3 ELISA kit
C7orf1 ELISA kit
CPETR2 ELISA kit
Specificity This kit recognizes Human CLDN3 in samples. No significant cross-reactivity or interference between Human CLDN3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-99 91
EDTA plasma (n=8) 88-101 93
Cell culture media (n=8) 89-101 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.99 2.88 7.66 1.04 2.90 8.21
Standard deviation 0.06 0.16 0.28 0.07 0.12 0.44
CV (%) 6.06 5.56 3.66 6.73 4.14 5.36
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance