| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.08 pg/mL |
| Detection Limit: |
15.63-1000 pg/mL |
| Short Description: |
This CHI3L1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CHI3L1 |
| Gene ID: |
1116 |
| Uniprot ID: |
CH3L1_HUMAN |
| Specificity: |
This kit recognizes Human CHI3L1 in samples. No significant cross-reactivity or interference between Human CHI3L1 and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Very low or undetectable expression in non-inflammatory colon. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts. |
| Post Translational Modifications | Glycosylated. |
| Function | Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung. |
| Protein Name | Chitinase-3-Like Protein 139 Kda Synovial ProteinCartilage Glycoprotein 39Cgp-39Gp-39Hcgp-39Ykl-40 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | SecretedExtracellular SpaceCytoplasmPerinuclear RegionEndoplasmic Reticulum |
| Alternative ELISA Names | Chitinase-3-Like Protein 1 ELISA kit39 Kda Synovial Protein ELISA kitCartilage Glycoprotein 39 ELISA kitCgp-39 ELISA kitGp-39 ELISA kitHcgp-39 ELISA kitYkl-40 ELISA kitCHI3L1 ELISA kit |
| Specificity | This kit recognizes Human CHI3L1 in samples. No significant cross-reactivity or interference between Human CHI3L1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-102 | 95 |
| EDTA plasma (n=8) | 89-102 | 95 |
| Cell culture media (n=8) | 87-100 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 53.96 | 125.80 | 399.76 | 52.35 | 117.62 | 392.92 |
| Standard deviation | 3.57 | 7.47 | 18.39 | 2.77 | 4.73 | 16.97 |
| CV (%) | 6.62 | 5.94 | 4.60 | 5.30 | 4.02 | 4.32 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
