Human CFD (Complement factor D) Sandwich ELISA (STJE0004019)

SKU:
STJE0004019

Shipping:
Free Shipping
Current Stock:
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.94ng/mL
Detection Limit: 1.56~100ng/mL
Short Description: This CFD Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CFD
Gene ID: 1675
Uniprot ID: CFAD_HUMAN
Specificity: This kit recognizes Human CFD in samples. No significant cross-reactivity or interference between Human CFD and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Complement Factor D
Adipsin
C3 Convertase Activator
Properdin Factor D
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-173736
Reactome: R-HSA-6798695
Cellular Localisation Secreted
Alternative ELISA Names Complement Factor D ELISA kit
Adipsin ELISA kit
C3 Convertase Activator ELISA kit
Properdin Factor D ELISA kit
CFD ELISA kit
DF ELISA kit
PFD ELISA kit
Specificity This kit recognizes Human CFD in samples. No significant cross-reactivity or interference between Human CFD and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-106 100
EDTA plasma (n=8) 97-106 102
Cell culture media (n=8) 93-100 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 4.66 11.33 46.40 4.89 12.36 41.26
Standard deviation 0.28 0.57 1.92 0.24 0.60 2.12
CV (%) 6.01 5.03 4.14 4.91 4.85 5.14
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance