| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This human CFD kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CFD |
| Gene ID: |
1675 |
| Uniprot ID: |
CFAD_HUMAN |
| Specificity: |
This kit recognizes Human CFD in samples. No significant cross-reactivity or interference between Human CFD and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. |
| Protein Name | Complement Factor DAdipsinC3 Convertase ActivatorProperdin Factor D |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-173736Reactome: R-HSA-6798695 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Complement Factor D CLIA kitAdipsin CLIA kitC3 Convertase Activator CLIA kitProperdin Factor D CLIA kitCFD CLIA kitDF CLIA kitPFD CLIA kit |
| Specificity | This kit recognizes Human CFD in samples. No significant cross-reactivity or interference between Human CFD and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 96-111 | 103 |
| EDTA plasma(n=8) | 84-95 | 90 |
| Cell culture media(n=8) | 100-116 | 107 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 233.24 | 687.31 | 2486.95 | 242.57 | 721.32 | 2632.72 |
| Standard deviation | 22.39 | 49.56 | 250.68 | 31.39 | 85.19 | 235.89 |
| CV (%) | 9.60 | 7.21 | 10.08 | 12.94 | 11.81 | 8.96 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
