| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human CDH13 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CDH13 |
| Gene ID: |
1012 |
| Uniprot ID: |
CAD13_HUMAN |
| Specificity: |
This kit recognizes Human H-Cadherin in samples. No significant cross-reactivity or interference between Human H-Cadherin and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Highly expressed in heart. In the CNS, expressed in cerebral cortex, medulla, hippocampus, amygdala, thalamus and substantia nigra. No expression detected in cerebellum or spinal cord. |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth. |
| Protein Name | Cadherin-13Heart CadherinH-CadherinP105Truncated CadherinT-CadT-Cadherin |
| Database Links | Reactome: R-HSA-418990 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-Anchor |
| Alternative CLIA Names | Cadherin-13 CLIA kitHeart Cadherin CLIA kitH-Cadherin CLIA kitP105 CLIA kitTruncated Cadherin CLIA kitT-Cad CLIA kitT-Cadherin CLIA kitCDH13 CLIA kitCDHH CLIA kit |
| Specificity | This kit recognizes Human H-Cadherin in samples. No significant cross-reactivity or interference between Human H-Cadherin and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-96 | 91 |
| EDTA plasma(n=8) | 91-105 | 98 |
| Cell culture media(n=8) | 87-98 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 99.47 | 251.28 | 887.18 | 107.43 | 248.23 | 974.77 |
| Standard deviation | 12.39 | 24.65 | 97.06 | 11.18 | 23.51 | 62.48 |
| CV (%) | 12.46 | 9.81 | 10.94 | 10.41 | 9.47 | 6.41 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
