• Human CDC25 (Cell Division Cycle Protein 25) Sandwich ELISA Kit (STJE0007828)

Human CDC25 (Cell Division Cycle Protein 25) Sandwich ELISA Kit (STJE0007828)

SKU:
STJE0007828

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.062ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This CDC25 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: CDC25A
Gene ID: 993
Uniprot ID: MPIP1_HUMAN
Sample Type: serum, plasma, tissue homogenates or other biological fluids.
Tissue Specificity
Post Translational Modifications Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation. Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
Function Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Protein Name M-Phase Inducer Phosphatase 1
Dual Specificity Phosphatase Cdc25a
Database Links Reactome: R-HSA-1362277
Reactome: R-HSA-156711
Reactome: R-HSA-176187
Reactome: R-HSA-5689880
Reactome: R-HSA-69202
Reactome: R-HSA-69273
Reactome: R-HSA-69601
Reactome: R-HSA-69656
Reactome: R-HSA-8862803
Cellular Localisation
Alternative ELISA Names M-Phase Inducer Phosphatase 1 ELISA kit
Dual Specificity Phosphatase Cdc25a ELISA kit
CDC25A ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance