| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human CD44 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CD44 |
| Gene ID: |
960 |
| Uniprot ID: |
CD44_HUMAN |
| Specificity: |
This kit recognizes Human HSPG in samples. No significant cross-reactivity or interference between Human HSPG and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Detected in fibroblasts and urine (at protein level). Detected in placenta (at protein level). Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. |
| Post Translational Modifications | Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. N-glycosylated. O-glycosylated.contains chondroitin sulfate glycans which can be more or less sulfated and whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638. Phosphorylated.activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. |
| Function | Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment. Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection. Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases. Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion. |
| Protein Name | Cd44 AntigenCdw44EpicanExtracellular Matrix Receptor IiiEcmr-IiiGp90 Lymphocyte Homing/Adhesion ReceptorHutch-IHeparan Sulfate ProteoglycanHermes AntigenHyaluronate ReceptorPhagocytic Glycoprotein 1Pgp-1Phagocytic Glycoprotein IPgp-ICd Antigen Cd44 |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-202733Reactome: R-HSA-216083Reactome: R-HSA-2160916Reactome: R-HSA-6798695Reactome: R-HSA-877300 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionMicrovillusSecretedColocalizes With Actin In Membrane Protrusions At Wounding EdgesCo-Localizes With RdxEzr And Msn In MicrovilliLocalizes To Cholesterol-Rich Membrane-Bound Lipid Raft Domains |
| Alternative CLIA Names | Cd44 Antigen CLIA kitCdw44 CLIA kitEpican CLIA kitExtracellular Matrix Receptor Iii CLIA kitEcmr-Iii CLIA kitGp90 Lymphocyte Homing/Adhesion Receptor CLIA kitHutch-I CLIA kitHeparan Sulfate Proteoglycan CLIA kitHermes Antigen CLIA kitHyaluronate Receptor CLIA kitPhagocytic Glycoprotein 1 CLIA kitPgp-1 CLIA kitPhagocytic Glycoprotein I CLIA kitPgp-I CLIA kitCd Antigen Cd44 CLIA kitCD44 CLIA kitLHR CLIA kitMDU2 CLIA kitMDU3 CLIA kitMIC4 CLIA kit |
| Specificity | This kit recognizes Human HSPG in samples. No significant cross-reactivity or interference between Human HSPG and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-99 | 92 |
| EDTA plasma(n=8) | 85-98 | 91 |
| Cell culture media(n=8) | 84-99 | 90 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 201.22 | 361.48 | 1532.83 | 194.74 | 332.89 | 1559.48 |
| Standard deviation | 19.82 | 31.63 | 155.12 | 17.96 | 28.96 | 134.27 |
| CV (%) | 9.85 | 8.75 | 10.12 | 9.22 | 8.70 | 8.61 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
