• Human CD36 (Platelet glycoprotein 4) CLIA Kit (STJC0000498)

Human CD36 (Platelet glycoprotein 4) CLIA Kit (STJC0000498)

SKU:
STJC0000498

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human CD36 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CD36
Gene ID: 948
Uniprot ID: CD36_HUMAN
Specificity: This kit recognizes Human GP4/CD36 in samples. No significant cross-reactivity or interference between Human GP4/CD36 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications N-glycosylated and O-glycosylated with a ratio of 2:1. Palmitoylated by ZDHHC5. Palmitoylation is required for proper localization at the plasma membrane. Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome. Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids.
Function Multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption. Mechanistically, binding of fatty acids activates downstream kinase LYN, which phosphorylates the palmitoyltransferase ZDHHC5 and inactivates it, resulting in the subsequent depalmitoylation of CD36 and caveolar endocytosis. In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway. Involved in oral fat perception and preferences. Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions. In taste receptor cells, mediates the induction of an increase in intracellular calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract. Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis. Receptor for thrombospondins, THBS1 and THBS2, mediating their antiangiogenic effects. Involved in inducing apoptosis in podocytes in response to elevated free fatty acids, acting together with THBS1. As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome. Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway. (Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling.
Protein Name Platelet Glycoprotein 4
Fatty Acid Translocase
Fat
Glycoprotein Iiib
Gpiiib
Leukocyte Differentiation Antigen Cd36
Pas Iv
Pas-4
Platelet Collagen Receptor
Platelet Glycoprotein Iv
Gpiv
Thrombospondin Receptor
Cd Antigen Cd36
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1236973
Reactome: R-HSA-1236974
Reactome: R-HSA-166058
Reactome: R-HSA-168188
Reactome: R-HSA-1989781
Reactome: R-HSA-3000471
Reactome: R-HSA-381340
Reactome: R-HSA-434313
Reactome: R-HSA-5602498
Reactome: R-HSA-5603041
Reactome: R-HSA-5686938
Reactome: R-HSA-6785807
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Membrane Raft
Golgi Apparatus
Apical Cell Membrane
Upon Ligand-Binding
Internalized Through Dynamin-Dependent Endocytosis
Alternative CLIA Names Platelet Glycoprotein 4 CLIA kit
Fatty Acid Translocase CLIA kit
Fat CLIA kit
Glycoprotein Iiib CLIA kit
Gpiiib CLIA kit
Leukocyte Differentiation Antigen Cd36 CLIA kit
Pas Iv CLIA kit
Pas-4 CLIA kit
Platelet Collagen Receptor CLIA kit
Platelet Glycoprotein Iv CLIA kit
Gpiv CLIA kit
Thrombospondin Receptor CLIA kit
Cd Antigen Cd36 CLIA kit
CD36 CLIA kit
GP3B CLIA kit
GP4 CLIA kit
Specificity This kit recognizes Human GP4/CD36 in samples. No significant cross-reactivity or interference between Human GP4/CD36 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-108 99
EDTA plasma(n=8) 84-99 91
Cell culture media(n=8) 91-102 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 100.52 221.67 819.66 92.55 236.73 831.41
Standard deviation 11.51 23.81 68.20 9.23 20.83 91.54
CV (%) 11.45 10.74 8.32 9.97 8.80 11.01
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance