| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.32 pg/mL |
| Detection Limit: |
78.13-5000 pg/mL |
| Short Description: |
This CD23 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
FCER2 |
| Gene ID: |
2208 |
| Uniprot ID: |
FCER2_HUMAN |
| Specificity: |
This kit recognizes Human CD23 in samples. No significant cross-reactivity or interference between Human CD23 and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Post Translational Modifications | N- and O-glycosylated. The secreted form sCD23 is produced by ADAM10-mediated ectodomain shedding. |
| Function | Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B cells. On B cells, initiates IgE-dependent antigen uptake and presentation to T cells. On macrophages, upon IgE binding and antigen cross-linking induces intracellular killing of parasites through activation of L-Arginine-nitric oxide pathway. |
| Protein Name | Low Affinity Immunoglobulin Epsilon Fc ReceptorBlast-2C-Type Lectin Domain Family 4 Member JFc-Epsilon-RiiImmunoglobulin E-Binding FactorLymphocyte Ige ReceptorCd Antigen Cd23 Cleaved Into - Low Affinity Immunoglobulin Epsilon Fc Receptor Membrane-Bound Form - Low Affinity Immunoglobulin Epsilon Fc Receptor Soluble Form |
| Database Links | Reactome: R-HSA-2197563Reactome: R-HSA-6783783Reactome: R-HSA-6785807 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinLipid-AnchorSecretedAlso Exists As A Soluble Excreted FormScd23 |
| Alternative ELISA Names | Low Affinity Immunoglobulin Epsilon Fc Receptor ELISA kitBlast-2 ELISA kitC-Type Lectin Domain Family 4 Member J ELISA kitFc-Epsilon-Rii ELISA kitImmunoglobulin E-Binding Factor ELISA kitLymphocyte Ige Receptor ELISA kitCd Antigen Cd23 Cleaved Into - Low Affinity Immunoglobulin Epsilon Fc Receptor Membrane-Bound Form - Low Affinity Immunoglobulin Epsilon Fc Receptor Soluble Form ELISA kitFCER2 ELISA kitCD23A ELISA kitCLEC4J ELISA kitFCE2 ELISA kitIGEBF ELISA kit |
| Specificity | This kit recognizes Human CD23 in samples. No significant cross-reactivity or interference between Human CD23 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-103 | 95 |
| EDTA plasma (n=8) | 94-111 | 102 |
| Cell culture media (n=8) | 87-100 | 92 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 254.15 | 496.51 | 2391.82 | 240.95 | 539.50 | 2433.85 |
| Standard deviation | 13.77 | 19.96 | 74.62 | 16.87 | 30.75 | 77.88 |
| CV (%) | 5.42 | 4.02 | 3.12 | 7.00 | 5.70 | 3.20 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
