| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This CCN4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
CCN4 |
| Gene ID: |
8840 |
| Uniprot ID: |
CCN4_HUMAN |
| Specificity: |
This kit recognizes Human WISP1 in samples. No significant cross-reactivity or interference between Human WISP1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. |
| Protein Name | Ccn Family Member 4Wnt1-Inducible-Signaling Pathway Protein 1Wisp-1Wnt-1-Induced Secreted Protein |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Ccn Family Member 4 ELISA kitWnt1-Inducible-Signaling Pathway Protein 1 ELISA kitWisp-1 ELISA kitWnt-1-Induced Secreted Protein ELISA kitCCN4 ELISA kitWISP1 ELISA kit |
| Specificity | This kit recognizes Human WISP1 in samples. No significant cross-reactivity or interference between Human WISP1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-101 | 94 |
| EDTA plasma (n=8) | 86-99 | 93 |
| Cell culture media (n=8) | 93-108 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 227.76 | 766.45 | 2132.87 | 205.65 | 798.91 | 2247.01 |
| Standard deviation | 11.48 | 37.25 | 83.18 | 10.82 | 33.23 | 68.98 |
| CV (%) | 5.04 | 4.86 | 3.90 | 5.26 | 4.16 | 3.07 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
