• Human CCN1 (CCN family member 1) Sandwich ELISA (STJE0002854)
  • Human CCN1 (CCN family member 1) Sandwich ELISA (STJE0002854)

Human CCN1 (CCN family member 1) Sandwich ELISA (STJE0002854)

SKU:
STJE0002854

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This CCN1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CCN1
Gene ID: 3491
Uniprot ID: CCN1_HUMAN
Specificity: This kit recognizes Human Cyr61 in samples. No significant cross-reactivity or interference between Human Cyr61 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Ccn Family Member 1
Cellular Communication Network Factor 1
Cysteine-Rich Angiogenic Inducer 61
Insulin-Like Growth Factor-Binding Protein 10
Ibp-10
Igf-Binding Protein 10
Igfbp-10
Protein Cyr61
Protein Gig1
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Alternative ELISA Names Ccn Family Member 1 ELISA kit
Cellular Communication Network Factor 1 ELISA kit
Cysteine-Rich Angiogenic Inducer 61 ELISA kit
Insulin-Like Growth Factor-Binding Protein 10 ELISA kit
Ibp-10 ELISA kit
Igf-Binding Protein 10 ELISA kit
Igfbp-10 ELISA kit
Protein Cyr61 ELISA kit
Protein Gig1 ELISA kit
CCN1 ELISA kit
CYR61 ELISA kit
GIG1 ELISA kit
IGFBP10 ELISA kit
Specificity This kit recognizes Human Cyr61 in samples. No significant cross-reactivity or interference between Human Cyr61 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-102 93
EDTA plasma (n=8) 91-103 98
Cell culture media (n=8) 91-103 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 101.37 188.28 957.65 106.56 172.93 978.96
Standard deviation 6.18 11.26 43.00 6.47 9.36 38.77
CV (%) 6.10 5.98 4.49 6.07 5.41 3.96
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance