• Human CCL8 (C-C motif chemokine 8) CLIA Kit (STJC0000541)

Human CCL8 (C-C motif chemokine 8) CLIA Kit (STJC0000541)

SKU:
STJC0000541

£238.50 - £477.50
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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 7.5pg/mL
Detection Limit: 12.5~800pg/mL
Short Description: This human CCL8 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CCL8
Gene ID: 6355
Uniprot ID: CCL8_HUMAN
Specificity: This kit recognizes Human MCP-2/CCL8 in samples. No significant cross-reactivity or interference between Human MCP-2/CCL8 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.
Post Translational Modifications N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
Function Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.
Protein Name C-C Motif Chemokine 8
Hc14
Monocyte Chemoattractant Protein 2
Monocyte Chemotactic Protein 2
Mcp-2
Small-Inducible Cytokine A8 Cleaved Into - Mcp-2(6-76
Cellular Localisation Secreted
Alternative CLIA Names C-C Motif Chemokine 8 CLIA kit
Hc14 CLIA kit
Monocyte Chemoattractant Protein 2 CLIA kit
Monocyte Chemotactic Protein 2 CLIA kit
Mcp-2 CLIA kit
Small-Inducible Cytokine A8 Cleaved Into - Mcp-2(6-76 CLIA kit
CCL8 CLIA kit
MCP2 CLIA kit
SCYA10 CLIA kit
SCYA8 CLIA kit
Specificity This kit recognizes Human MCP-2/CCL8 in samples. No significant cross-reactivity or interference between Human MCP-2/CCL8 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 97-113 105
EDTA plasma(n=8) 98-110 105
Cell culture media(n=8) 99-117 106

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 43.46 114.48 306.04 45.21 106.03 277.17
Standard deviation 4.80 10.36 28.16 4.77 8.20 27.33
CV (%) 11.04 9.05 9.20 10.55 7.73 9.86
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance