Applications: |
CLIA |
Reactivity: |
Human |
Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: |
9.38pg/mL |
Detection Limit: |
15.63~1000pg/mL |
Short Description: |
This human CCL3L1; CCL3L3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: |
3.5h |
Detection: |
Chemiluminescence |
Gene Symbol: |
CCL3L1.CCL3L3 |
Gene ID: |
414062/6349 |
Uniprot ID: |
CL3L1_HUMAN |
Specificity: |
This kit recognizes Human CCL3L1 in samples. No significant cross-reactivity or interference between Human CCL3L1 and analogues was observed. |
Sample Type: |
Serum, plasma and other biological fluids |
Post Translational Modifications | The N-terminal processed forms LD78-beta(3-70) and LD78-beta(5-70) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. The cleavage to yield LD78-beta(3-70) is probably achieved by DPP4. |
Function | Chemotactic for lymphocytes and monocytes. Is a ligand for CCR1, CCR3 and CCR5. Is an inhibitor of HIV-1-infection. The processed form LD78-beta(3-70) shows a 20-fold to 30-fold higher chemotactic activity and is a very potent inhibitor of HIV-1-infection. LD78-beta(3-70) is also a ligand for CCR1, CCR3 and CCR5. |
Protein Name | C-C Motif Chemokine 3-Like 1G0/G1 Switch Regulatory Protein 19-2Ld78-Beta(1-70Pat 464.2Small-Inducible Cytokine A3-Like 1Tonsillar Lymphocyte Ld78 Beta Protein Cleaved Into - Ld78-Beta(3-70 - Ld78-Beta(5-70 |
Database Links | Reactome: R-HSA-380108Reactome: R-HSA-6783783 |
Cellular Localisation | Secreted |
Alternative CLIA Names | C-C Motif Chemokine 3-Like 1 CLIA kitG0/G1 Switch Regulatory Protein 19-2 CLIA kitLd78-Beta(1-70 CLIA kitPat 464.2 CLIA kitSmall-Inducible Cytokine A3-Like 1 CLIA kitTonsillar Lymphocyte Ld78 Beta Protein Cleaved Into - Ld78-Beta(3-70 - Ld78-Beta(5-70 CLIA kitCCL3L1 CLIA kitD17S1718 CLIA kitG0S19-2 CLIA kitSCYA3L1.CCL3L3 CLIA kit |
Specificity | This kit recognizes Human CCL3L1 in samples. No significant cross-reactivity or interference between Human CCL3L1 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 97-110 | 103 |
EDTA plasma(n=8) | 87-98 | 92 |
Cell culture media(n=8) | 98-111 | 103 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 49.77 | 98.67 | 402.56 | 45.01 | 92.77 | 413.15 |
Standard deviation | 4.15 | 10.19 | 44.89 | 4.58 | 8.12 | 43.46 |
CV (%) | 8.34 | 10.33 | 11.15 | 10.18 | 8.75 | 10.52 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance