| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human CCL21 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CCL21 |
| Gene ID: |
6366 |
| Uniprot ID: |
CCL21_HUMAN |
| Specificity: |
This kit recognizes Human SLC in samples. No significant cross-reactivity or interference between Human SLC and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen. |
| Function | Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. |
| Protein Name | C-C Motif Chemokine 216ckineBeta-Chemokine Exodus-2Secondary Lymphoid-Tissue ChemokineSlcSmall-Inducible Cytokine A21 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-418594 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | C-C Motif Chemokine 21 CLIA kit6ckine CLIA kitBeta-Chemokine Exodus-2 CLIA kitSecondary Lymphoid-Tissue Chemokine CLIA kitSlc CLIA kitSmall-Inducible Cytokine A21 CLIA kitCCL21 CLIA kitSCYA21 CLIA kitUNQ784 CLIA kitPRO1600 CLIA kit |
| Specificity | This kit recognizes Human SLC in samples. No significant cross-reactivity or interference between Human SLC and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 99-111 | 105 |
| EDTA plasma(n=8) | 87-100 | 94 |
| Cell culture media(n=8) | 89-104 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 95.97 | 200.41 | 874.20 | 99.02 | 195.18 | 955.07 |
| Standard deviation | 10.41 | 20.58 | 95.38 | 8.17 | 14.97 | 60.65 |
| CV (%) | 10.85 | 10.27 | 10.91 | 8.25 | 7.67 | 6.35 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
