| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This human CCL14 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CCL14 |
| Gene ID: |
6358 |
| Uniprot ID: |
CCL14_HUMAN |
| Specificity: |
This kit recognizes Human CCL14 in samples. No significant cross-reactivity or interference between Human CCL14 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations (1-80 nM) in plasma. |
| Post Translational Modifications | The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood. HCC-1(1-74), but not HCC-1(3-74) and HCC-1(4-74), is partially O-glycosylated.the O-linked glycan consists of one Gal-GalNAc disaccharide, further modified by two N-acetylneuraminic acids. |
| Function | Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induces intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and is inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes, eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5. |
| Protein Name | C-C Motif Chemokine 14Chemokine Cc-1/Cc-3Hcc-1/Hcc-3Hcc-1(1-74Ncc-2Small-Inducible Cytokine A14 Cleaved Into - Hcc-1(3-74 - Hcc-1(4-74 - Hcc-1(9-74 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | C-C Motif Chemokine 14 CLIA kitChemokine Cc-1/Cc-3 CLIA kitHcc-1/Hcc-3 CLIA kitHcc-1(1-74 CLIA kitNcc-2 CLIA kitSmall-Inducible Cytokine A14 Cleaved Into - Hcc-1(3-74 - Hcc-1(4-74 - Hcc-1(9-74 CLIA kitCCL14 CLIA kitNCC2 CLIA kitSCYA14 CLIA kit |
| Specificity | This kit recognizes Human CCL14 in samples. No significant cross-reactivity or interference between Human CCL14 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-103 | 95 |
| EDTA plasma(n=8) | 89-102 | 95 |
| Cell culture media(n=8) | 86-100 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 39.77 | 127.62 | 317.92 | 38.63 | 118.11 | 324.41 |
| Standard deviation | 3.47 | 11.29 | 22.00 | 3.64 | 9.73 | 26.80 |
| CV (%) | 8.73 | 8.85 | 6.92 | 9.42 | 8.24 | 8.26 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
