Applications: |
CLIA |
Reactivity: |
Human |
Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: |
9.38pg/mL |
Detection Limit: |
15.63~1000pg/mL |
Short Description: |
This human CACYBP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: |
3.5h |
Detection: |
Chemiluminescence |
Gene Symbol: |
CACYBP |
Gene ID: |
27101 |
Uniprot ID: |
CYBP_HUMAN |
Specificity: |
This kit recognizes Human CACYBP in samples. No significant cross-reactivity or interference between Human CACYBP and analogues was observed. |
Sample Type: |
Serum, plasma and other biological fluids |
Post Translational Modifications | Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations. |
Function | May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1). |
Protein Name | Calcyclin-Binding ProteinCacybpHcacybpS100a6-Binding ProteinSiah-Interacting Protein |
Cellular Localisation | NucleusCytoplasmCytoplasmic At Low Calcium ConcentrationsIn Neuroblastoma CellsAfter A Retinoic Acid (Ra) Induction And Calcium IncreaseIt Localizes In Both The Nucleus And CytoplasmThe Nuclear Fraction May Be Phosphorylated |
Alternative CLIA Names | Calcyclin-Binding Protein CLIA kitCacybp CLIA kitHcacybp CLIA kitS100a6-Binding Protein CLIA kitSiah-Interacting Protein CLIA kitCACYBP CLIA kitS100A6BP CLIA kitSIP CLIA kitPNAS-107 CLIA kit |
Specificity | This kit recognizes Human CACYBP in samples. No significant cross-reactivity or interference between Human CACYBP and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 94-107 | 100 |
EDTA plasma(n=8) | 99-114 | 105 |
Cell culture media(n=8) | 93-105 | 98 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 50.04 | 130.84 | 357.68 | 52.13 | 142.16 | 351.70 |
Standard deviation | 5.54 | 14.17 | 22.14 | 5.95 | 12.69 | 34.33 |
CV (%) | 11.07 | 10.83 | 6.19 | 11.41 | 8.93 | 9.76 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance