| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.38ng/mL |
| Detection Limit: |
0.63~40ng/mL |
| Short Description: |
This human CA2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
CA2 |
| Gene ID: |
760 |
| Uniprot ID: |
CAH2_HUMAN |
| Specificity: |
This kit recognizes Human CA2 in samples. No significant cross-reactivity or interference between Human CA2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Catalyzes the reversible hydration of carbon dioxide. Can also hydrate cyanamide to urea. Stimulates the chloride-bicarbonate exchange activity of SLC26A6. Essential for bone resorption and osteoclast differentiation. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. |
| Protein Name | Carbonic Anhydrase 2Carbonate Dehydratase IiCarbonic Anhydrase CCacCarbonic Anhydrase IiCa-IiCyanamide Hydratase Ca2 |
| Database Links | Reactome: R-HSA-1237044Reactome: R-HSA-1247673Reactome: R-HSA-1475029 |
| Cellular Localisation | CytoplasmCell MembraneColocalized With Slc26a6 At The Surface Of The Cell Membrane In Order To Form A Bicarbonate Transport MetabolonDisplaced From The Cytosolic Surface Of The Cell Membrane By Pkc In Phorbol Myristate Acetate (Pma)-Induced Cells |
| Alternative CLIA Names | Carbonic Anhydrase 2 CLIA kitCarbonate Dehydratase Ii CLIA kitCarbonic Anhydrase C CLIA kitCac CLIA kitCarbonic Anhydrase Ii CLIA kitCa-Ii CLIA kitCyanamide Hydratase Ca2 CLIA kitCA2 CLIA kit |
| Specificity | This kit recognizes Human CA2 in samples. No significant cross-reactivity or interference between Human CA2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-106 | 101 |
| EDTA plasma(n=8) | 95-109 | 102 |
| Cell culture media(n=8) | 90-101 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.00 | 5.04 | 15.04 | 2.14 | 5.40 | 16.47 |
| Standard deviation | 0.17 | 0.47 | 1.51 | 0.25 | 0.57 | 1.15 |
| CV (%) | 8.50 | 9.33 | 10.04 | 11.68 | 10.56 | 6.98 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
