| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.124ng/mL |
| Detection Limit: | 0.312-20ng/mL |
| Short Description: | This BRAF Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | BRAF |
| Gene ID: | 673 |
| Uniprot ID: | BRAF_HUMAN |
| Sample Type: | tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Brain and testis. |
| Post Translational Modifications | Phosphorylation at Ser-365 by SGK1 inhibits its activity. Methylation at Arg-671 decreases stability and kinase activity. Ubiquitinated by RNF149.which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF. |
| Function | Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. Phosphorylates PFKFB2. May play a role in the postsynaptic responses of hippocampal neurons. |
| Protein Name | Serine/Threonine-Protein Kinase B-RafProto-Oncogene B-RafP94V-Raf Murine Sarcoma Viral Oncogene Homolog B1 |
| Database Links | Reactome: R-HSA-1295596Reactome: R-HSA-170968Reactome: R-HSA-170984Reactome: R-HSA-187706Reactome: R-HSA-5673000Reactome: R-HSA-5674135Reactome: R-HSA-5674499Reactome: R-HSA-5675221Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-9649948Reactome: R-HSA-9656223Reactome: R-HSA-9726840Reactome: R-HSA-9726842 |
| Cellular Localisation | NucleusCytoplasmCell MembraneColocalizes With Rgs14 And Raf1 In Both The Cytoplasm And Membranes |
| Alternative ELISA Names | Serine/Threonine-Protein Kinase B-Raf ELISA kitProto-Oncogene B-Raf ELISA kitP94 ELISA kitV-Raf Murine Sarcoma Viral Oncogene Homolog B1 ELISA kitBRAF ELISA kitBRAF1 ELISA kitRAFB1 ELISA kit |
| output |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
