• Human BRAF (B-Raf Proto Oncogene Serine/Threonine Protein Kinase) Sandwich ELISA Kit Ready-To-Use (STJE0013917)

Human BRAF (B-Raf Proto Oncogene Serine/Threonine Protein Kinase) Sandwich ELISA Kit Ready-To-Use (STJE0013917)

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STJE0013917

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.124ng/mL
Detection Limit: 0.312-20ng/mL
Short Description: This BRAF Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: BRAF
Gene ID: 673
Uniprot ID: BRAF_HUMAN
Immunogen Region: Ready-To-Use
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity Brain and testis.
Post Translational Modifications Phosphorylation at Ser-365 by SGK1 inhibits its activity. Methylation at Arg-671 decreases stability and kinase activity. Ubiquitinated by RNF149.which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.
Function Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. Phosphorylates PFKFB2. May play a role in the postsynaptic responses of hippocampal neurons.
Protein Name Serine/Threonine-Protein Kinase B-Raf
Proto-Oncogene B-Raf
P94
V-Raf Murine Sarcoma Viral Oncogene Homolog B1
Database Links Reactome: R-HSA-1295596
Reactome: R-HSA-170968
Reactome: R-HSA-170984
Reactome: R-HSA-187706
Reactome: R-HSA-5673000
Reactome: R-HSA-5674135
Reactome: R-HSA-5674499
Reactome: R-HSA-5675221
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9726840
Reactome: R-HSA-9726842
Cellular Localisation Nucleus
Cytoplasm
Cell Membrane
Colocalizes With Rgs14 And Raf1 In Both The Cytoplasm And Membranes
Alternative ELISA Names Serine/Threonine-Protein Kinase B-Raf ELISA kit
Proto-Oncogene B-Raf ELISA kit
P94 ELISA kit
V-Raf Murine Sarcoma Viral Oncogene Homolog B1 ELISA kit
BRAF ELISA kit
BRAF1 ELISA kit
RAFB1 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance