| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This BCL2L1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
BCL2L1 |
| Gene ID: |
598 |
| Uniprot ID: |
B2CL1_HUMAN |
| Specificity: |
This kit recognizes Human BCL2L1/BCL-X in samples. No significant cross-reactivity or interference between Human BCL2L1/BCL-X and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity. Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis. Ubiquitinated by RNF183 during prolonged ER stress, leading to degradation by the proteosome. |
| Function | Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. Isoform Bcl-X(S) promotes apoptosis. |
| Protein Name | Bcl-2-Like Protein 1Bcl2-L-1Apoptosis Regulator Bcl-X |
| Database Links | Reactome: R-HSA-111453Reactome: R-HSA-6785807Reactome: R-HSA-844455Reactome: R-HSA-9648002Reactome: R-HSA-9692913Reactome: R-HSA-9702518 |
| Cellular Localisation | Isoform Bcl-X(L): Mitochondrion Inner MembraneMitochondrion Outer MembraneMitochondrion MatrixCytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneCytoplasmCytosolCytoskeletonMicrotubule Organizing CenterCentrosomeNucleus MembraneSingle-Pass Membrane ProteinCytoplasmic SideAfter Neuronal StimulationTranslocates From Cytosol To Synaptic Vesicle And Mitochondrion Membrane In A Calmodulin-Dependent MannerLocalizes To The Centrosome When Phosphorylated At Ser-49 |
| Alternative ELISA Names | Bcl-2-Like Protein 1 ELISA kitBcl2-L-1 ELISA kitApoptosis Regulator Bcl-X ELISA kitBCL2L1 ELISA kitBCL2L ELISA kitBCLX ELISA kit |
| Specificity | This kit recognizes Human BCL2L1/BCL-X in samples. No significant cross-reactivity or interference between Human BCL2L1/BCL-X and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-101 | 96 |
| EDTA plasma (n=8) | 90-105 | 97 |
| Cell culture media (n=8) | 84-97 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.06 | 2.02 | 8.95 | 1.14 | 1.97 | 8.29 |
| Standard deviation | 0.06 | 0.11 | 0.27 | 0.06 | 0.08 | 0.36 |
| CV (%) | 5.66 | 5.45 | 3.02 | 5.26 | 4.06 | 4.34 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
