| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human BCAN kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
BCAN |
| Gene ID: |
63827 |
| Uniprot ID: |
PGCB_HUMAN |
| Specificity: |
This kit recognizes Human BCAN in samples. No significant cross-reactivity or interference between Human BCAN and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in the retina, specifically in the inner nuclear layer, inner plexiform layer and ganglion cell layer (at protein level). Detected in cerebrospinal fluid (at protein level). |
| Post Translational Modifications | O-glycosylated.contains chondroitin sulfate. O-glycosylated with a core 1 or possibly core 8 glycan. |
| Function | May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans. |
| Protein Name | Brevican Core ProteinBrain-Enriched Hyaluronan-Binding ProteinBehabChondroitin Sulfate Proteoglycan 7 |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-1971475Reactome: R-HSA-2022870Reactome: R-HSA-2022923Reactome: R-HSA-2024101Reactome: R-HSA-3000178Reactome: R-HSA-3560783Reactome: R-HSA-3560801Reactome: R-HSA-3595172Reactome: R-HSA-3595174Reactome: R-HSA-3595177Reactome: R-HSA-4420332 |
| Cellular Localisation | SecretedIsoform 1: SecretedExtracellular SpaceExtracellular MatrixIsoform 2: MembraneLipid-AnchorGpi-Anchor |
| Alternative CLIA Names | Brevican Core Protein CLIA kitBrain-Enriched Hyaluronan-Binding Protein CLIA kitBehab CLIA kitChondroitin Sulfate Proteoglycan 7 CLIA kitBCAN CLIA kitBEHAB CLIA kitCSPG7 CLIA kitUNQ2525 CLIA kitPRO6018 CLIA kit |
| Specificity | This kit recognizes Human BCAN in samples. No significant cross-reactivity or interference between Human BCAN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 101-114 | 108 |
| EDTA plasma(n=8) | 98-112 | 105 |
| Cell culture media(n=8) | 91-107 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 202.28 | 385.10 | 1447.18 | 201.64 | 346.96 | 1539.55 |
| Standard deviation | 19.54 | 40.78 | 142.84 | 18.21 | 36.85 | 136.40 |
| CV (%) | 9.66 | 10.59 | 9.87 | 9.03 | 10.62 | 8.86 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
